Wang Xiaoning, Zhang Changjun, Zhang Ying, Ru Xue, Gong Qinqin
Cencer of Reproductive Medicine, Affiliated People's Hospital of Hubei Medical College, Shiyan, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Nov;32(11):1643-5.
To evaluate the feasibility of establishing a mouse model of ovarian oxidative stress by intraperitoneal injections of arsenic sodium.
Twenty adult female Kunming mice were randomized equally into the normal control group and ovarian oxidative stress model group for intraperitoneal injections of 0.5 ml distilled water and 8 mg/kg arsenic sodium solution every other day, respectively. After 8 injections, the mice were sacrificed for histological observation of the ovarian sections and enzyme-linked immunosorbent assay (ELISA) of serum estradiol (E(2)) and pregnenedione (P) levels ande contents of reactive oxygen species (ROS) , malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in the ovary homogenate.
Numerous atretic follicles were found in the ovaries of mice in the model group with obviously reduced growing follicles. Compared with those in the normal control group, the contents of ROS and MDA increased and SOD and GSH-Px levels in the ovarian homogenate decreased significantly in the model group (P<0.05).
A mouse model of ovarian oxidative stress can be established by intraperitoneal injections of arsenic sodium.
评估通过腹腔注射砷酸钠建立小鼠卵巢氧化应激模型的可行性。
将20只成年雌性昆明小鼠随机均分为正常对照组和卵巢氧化应激模型组，分别隔日腹腔注射0.5 ml蒸馏水和8 mg/kg砷酸钠溶液。注射8次后，处死小鼠，对卵巢切片进行组织学观察，并采用酶联免疫吸附测定（ELISA）法检测血清雌二醇（E₂）、孕烯二酮（P）水平以及卵巢匀浆中活性氧（ROS）、丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）的含量。
模型组小鼠卵巢中可见大量闭锁卵泡，生长卵泡明显减少。与正常对照组相比，模型组卵巢匀浆中ROS和MDA含量增加，SOD和GSH-Px水平显著降低（P<0.05）。
腹腔注射砷酸钠可建立小鼠卵巢氧化应激模型。
