Department of Medicinal Chemistry, Key Laboratory of Chemical Biology of Natural Products (MOE), School of Pharmacy, Shandong University, Jinan, Shandong 250012, China.
Org Biomol Chem. 2013 Jan 14;11(2):378-82. doi: 10.1039/c2ob26564h. Epub 2012 Nov 23.
In the current paper, three activity-based colorimetric and ratiometric fluorescent probes based on a naphthalimide fluorophore were well designed and synthesized, which can be recognized and hydrolyzed by aminopeptidase N (APN) at both the enzymatic and cellular level by following the fluorescent emission wavelength change from blue to green light. As a result, these molecules were successfully identified as the first ratiometric fluorescent probes for APN cell imaging.
在当前的论文中，设计并合成了基于萘酰亚胺荧光团的三种基于活性的比色和比率荧光探针，它们可以通过酶和细胞水平上的氨肽酶 N (APN) 的识别和水解，根据荧光发射波长从蓝光到绿光的变化进行识别和水解。因此，这些分子被成功鉴定为用于 APN 细胞成像的第一个比率荧光探针。
