Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University , Jinan, Shandong 250012, China.
Anal Chem. 2014 Mar 4;86(5):2747-51. doi: 10.1021/ac404176x. Epub 2014 Feb 12.
To find an approach that can image the hydrolysis activity of aminopeptidase N (APN) both in vitro and in vivo, three bioluminescent probes have been well designed and synthesized herein. All of them can be recognized and hydrolyzed by APN to produce bioluminescence emission in the presence of firefly luciferase. To the best of our knowledge, they are the first bioluminescent probes for imaging APN in deep tissues and living animals.
为了寻找一种既能在体外又能在体内对氨基肽酶 N (APN) 的水解活性进行成像的方法，本文设计并合成了三种生物发光探针。在萤火虫荧光素的存在下，所有这些探针都可以被 APN 识别和水解，从而产生生物发光发射。据我们所知，它们是第一个用于在深部组织和活体动物中成像 APN 的生物发光探针。
