Arnao M B, Acosta M, del Río J A, García-Cánovas F
Departamento de Biología Vegetal (Fisiología Vegetal), Universidad de Murcia, Spain.
Biochim Biophys Acta. 1990 Mar 29;1038(1):85-9. doi: 10.1016/0167-4838(90)90014-7.
Hydrogen peroxide, the oxidant substrate of peroxidase, is also an inactivating agent of this enzyme. The reductant substrates protect the enzyme from the inactivating process. A reaction mechanism is proposed, in which two competitive routes exist for Compound I of peroxidase; one catalytic and one inactivating. The analytical solution produced at the end of the reaction supports the proposed mechanism and shows the dependence between the number of turnovers of the enzyme (r) and the ratio of both substrates.
过氧化氢作为过氧化物酶的氧化底物，同时也是该酶的一种失活剂。还原底物可保护该酶免受失活过程的影响。本文提出了一种反应机制，其中过氧化物酶的化合物I存在两条竞争途径；一条是催化途径，一条是失活途径。反应结束时得到的解析解支持了所提出的机制，并显示了酶的周转数（r）与两种底物比例之间的依赖性。
