School of Chemistry and Material Science, Shanxi Normal University, Linfen, 041004, P.R. China.
Luminescence. 2013 Nov-Dec;28(6):888-93. doi: 10.1002/bio.2452. Epub 2012 Nov 23.
A rapid and sensitive flow-injection chemiluminescence (FI-CL) method is described for the determination of diazepam based on its reaction with N-bromosuccinimide (NBS) in alkaline medium in the presence of dichlorofluorescein (DCF) as an effective energy-transfer agent. Under optimum conditions, the proposed method allowed the measurement of diazepam over the range of 2.0 × 10(-6) to 2.0 × 10(-4) mol/L with a detection limit of 5.0 × 10(-7) mol/L. The relative standard deviation for 11 parallel measurements of 2.0 × 10(-5) mol/L diazepam was 2.1%. The method was applied satisfactorily for the determination of diazepam in pharmaceutical preparations, and the results agree well with those obtained by spectrophotometry. The use of the proposed system for the determination of diazepam in urine and plasma samples was also tested. The possible mechanism of the chemiluminescence reaction is discussed briefly.
一种快速灵敏的流动注射化学发光（FI-CL）法，用于测定安定。该方法基于安定与 N-溴代琥珀酰亚胺（NBS）在碱性介质中的反应，在该反应中，二氯荧光素（DCF）作为一种有效的能量转移剂。在最佳条件下，该方法允许在 2.0×10(-6) 至 2.0×10(-4) mol/L 的范围内测量安定，检测限为 5.0×10(-7) mol/L。对于 2.0×10(-5) mol/L 安定的 11 次平行测量，相对标准偏差为 2.1%。该方法已成功应用于药物制剂中安定的测定，与分光光度法的结果吻合良好。还测试了该体系用于尿液和血浆样品中安定的测定。简要讨论了化学发光反应的可能机制。
