Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2013 Oct;114:33-7. doi: 10.1016/j.saa.2013.04.097. Epub 2013 May 7.
A simple, rapid and sensitive chemiluminescence (CL) method combined with flow injection analysis was developed for the determination of trimetazidine. Trimetazidine was found to significantly increase the CL signal arising from N-bromosuccinimide-luminol reaction in the presence of gold nanoparticles. The enhanced CL intensity was proportional to trimetazidine concentration in the range of 0.01-5.0 μg/mL, with a limit of detection (3 sb) of 6.7 ng/mL. The relative standard deviation was 2.8% for 11 repetitive measurements of 0.1 μg/mL trimetazidine solution. The practicality of the method was evaluated by determining trimetazidine in pharmaceutical formulations and in spiked human serum samples. Moreover, the possible CL reaction mechanism was also discussed.
建立了一种简单、快速、灵敏的化学发光（CL）分析法，用于测定曲美他嗪。在金纳米粒子存在下，发现曲美他嗪能显著增强 N-溴代丁二酰亚胺-鲁米诺反应的 CL 信号。增强的 CL 强度与曲美他嗪浓度在 0.01-5.0 μg/mL 范围内呈正比，检出限（3 sb）为 6.7 ng/mL。对 0.1 μg/mL 曲美他嗪溶液的 11 次重复测量，相对标准偏差为 2.8%。该方法用于测定药物制剂和人血清样品中的曲美他嗪，实用性良好。此外，还讨论了可能的 CL 反应机制。
