Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto 615-8510, Japan.
Curr Opin Chem Biol. 2012 Dec;16(5-6):609-13. doi: 10.1016/j.cbpa.2012.10.022. Epub 2012 Nov 21.
Isotope-assisted multi-dimensional NMR spectroscopy can now be applied to proteins inside living cells. The technique, called in-cell NMR, aims to investigate the structures, interactions and dynamics of proteins under their native conditions, ideally at an atomic resolution. The application has begun with bacterial cells but has now expanded to mammalian cultured cells, such as HeLa cells. The importance of the realization of such 'in-mammalian cell' NMR should be stressed, as these are the cells most often employed in cell biology. Hence, a substantially wide range of application would be possible in the near future once the technique has been well developed.
同位素辅助多维核磁共振波谱现在可以应用于活细胞内的蛋白质。这项技术被称为细胞内 NMR，旨在研究蛋白质在其天然环境下的结构、相互作用和动态，理想情况下达到原子分辨率。该应用最初是在细菌细胞中进行的，但现在已经扩展到哺乳动物培养细胞，如 HeLa 细胞。应该强调实现这种“哺乳动物细胞内”NMR 的重要性，因为这些细胞是细胞生物学中最常使用的细胞。因此，一旦该技术得到充分发展，在不久的将来，它将有广泛的应用。
