迈向动态结构生物学：单分子Förster 共振能量转移的二十年。
Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer.
机构信息
Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA.
Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen, 9747 AG Groningen, Netherlands.
出版信息
Science. 2018 Jan 19;359(6373). doi: 10.1126/science.aan1133.
Classical structural biology can only provide static snapshots of biomacromolecules. Single-molecule Förster resonance energy transfer (smFRET) paved the way for studying dynamics in macromolecular structures under biologically relevant conditions. Since its first implementation in 1996, smFRET experiments have confirmed previously hypothesized mechanisms and provided new insights into many fundamental biological processes, such as DNA maintenance and repair, transcription, translation, and membrane transport. We review 22 years of contributions of smFRET to our understanding of basic mechanisms in biochemistry, molecular biology, and structural biology. Additionally, building on current state-of-the-art implementations of smFRET, we highlight possible future directions for smFRET in applications such as biosensing, high-throughput screening, and molecular diagnostics.
经典结构生物学只能提供生物大分子的静态快照。单分子Förster 共振能量转移（smFRET）为在生物相关条件下研究大分子结构的动力学铺平了道路。自 1996 年首次实施以来，smFRET 实验已经证实了先前假设的机制，并为许多基本生物过程（如 DNA 维护和修复、转录、翻译和膜转运）提供了新的见解。我们回顾了 smFRET 在 22 年来对我们理解生物化学、分子生物学和结构生物学基本机制的贡献。此外，基于 smFRET 的当前最先进的实现，我们强调了 smFRET 在生物传感、高通量筛选和分子诊断等应用中的可能未来方向。
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