在活细胞中进行 RNA 构象分析。
RNA SHAPE analysis in living cells.
机构信息
Howard Hughes Medical Institute, Stanford University School of Medicine, Stanford, California, USA.
出版信息
Nat Chem Biol. 2013 Jan;9(1):18-20. doi: 10.1038/nchembio.1131. Epub 2012 Nov 25.
Abstract
RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE) in living cells. RNA structures in human, mouse, fly, yeast and bacterial cells are read out at single-nucleotide resolution, revealing tertiary contacts and RNA-protein interactions.
摘要
RNA 结构在基因调控的几乎各个方面都具有重要作用，但体内结构探针的缺乏限制了当前的理解。在这里，我们设计、合成并证明了两种新的化学探针，它们可以在活细胞中进行选择性 2'-羟基酰化分析引物延伸（SHAPE）。在人类、小鼠、果蝇、酵母和细菌细胞中读取 RNA 结构，分辨率达到单个核苷酸，揭示三级接触和 RNA-蛋白质相互作用。
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