日本腹泻儿童中普罗维登斯菌属菌株的流行情况。
Prevalence of Providencia strains among children with diarrhea in Japan.
机构信息
Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
出版信息
Jpn J Infect Dis. 2012;65(6):545-7. doi: 10.7883/yoken.65.545.
In the present study, we examined the prevalence of Providencia spp. strains among children with diarrhea in Japan. We developed a Providencia genus-specific polymerase chain reaction (PCR) method, and the specificity and sensitivity was evaluated to be 100% with various bacterial strains including 7 genera and 13 species. Five of 345 samples (1.4%) were positive by PCR using a Providencia genus-specific primer targeting the 16S rRNA gene. The single species Providencia rettgeri was isolated from 4 stool samples of children with diarrhea. The prevalence of Providencia spp. in children with diarrhea in Japan is lower than that previously reported for Japanese travelers abroad with diarrhea.
在本研究中，我们检测了日本腹泻儿童中普罗威登斯菌属菌株的流行情况。我们建立了普罗威登斯菌属特异性聚合酶链反应（PCR）方法，其对包括 7 个属和 13 个种在内的各种细菌菌株的特异性和敏感性均为 100%。使用针对 16S rRNA 基因的普罗威登斯菌属特异性引物进行 PCR 检测，345 个样本中有 5 个（1.4%）呈阳性。从 4 例腹泻患儿的粪便中分离出单一菌种雷特氏普罗威登斯菌。日本腹泻儿童中普罗威登斯菌属的流行率低于以往报道的日本腹泻旅行者。
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