Hatanaka Noritoshi, Shimizu Akinori, Somroop Srinuan, Li Yiming, Asakura Masahiro, Nagita Akira, Prasad Awasthi Sharda, Hinenoya Atsushi, Yamasaki Shinji
Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
School of Light Industry and Food Science, South China University of Technology.
Jpn J Infect Dis. 2017 Jul 24;70(4):455-457. doi: 10.7883/yoken.JJID.2016.428. Epub 2017 Feb 28.
Campylobacter ureolyticus has been considered as a potentially pathogenic bacterium. In this study, a total of 586 stool samples were collected from 0-12-year-old children with diarrhea between November 2013 and April 2015 and examined with microbiological tests in the hospital for the diagnosis of common enteric pathogens including C. jejuni and C. coli. Then in our laboratory, these samples were analyzed by 16S rRNA sequence-based Campylobacter genus-specific PCR (C16S PCR); 283 (48.3%) samples showed positive results with this PCR assay. Furthermore, C. ureolyticus was screened in these 283 samples by PCR assay, which can detect this species specifically. Surprisingly, C. ureolyticus was detected in 147 of the 283 C16S PCR-positive diarrheal stool samples (51.9%), which is much higher than the prevalence of C. jejuni and C. coli (15.5%), and 96 samples out of 147 were negative for any of the other enteric pathogens tested in the hospital; namely, C. ureolyticus was detected as a single pathogen in 96 samples. This finding suggests that C. ureolyticus may be a pathogen associated with diarrhea in children in Japan. To the best of our knowledge, this is the first report in which C. ureolyticus was detected among Japanese children with diarrhea.
解脲弯曲菌被认为是一种潜在的病原菌。在本研究中，于2013年11月至2015年4月期间，从0至12岁腹泻儿童中总共收集了586份粪便样本，并在医院进行微生物检测，以诊断包括空肠弯曲菌和结肠弯曲菌在内的常见肠道病原体。然后在我们实验室，通过基于16S rRNA序列的弯曲菌属特异性PCR（C16S PCR）对这些样本进行分析；该PCR检测中有283份（48.3%）样本呈阳性结果。此外，通过能特异性检测该菌种的PCR检测法，在这283份样本中筛查解脲弯曲菌。令人惊讶的是，在283份C16S PCR阳性腹泻粪便样本中有147份（51.9%）检测到了解脲弯曲菌，这远高于空肠弯曲菌和结肠弯曲菌的患病率（15.5%），并且在147份样本中有96份在医院检测的任何其他肠道病原体检测中均为阴性；也就是说，在96份样本中解脲弯曲菌被检测为单一病原体。这一发现表明解脲弯曲菌可能是日本儿童腹泻相关的一种病原体。据我们所知，这是在日本腹泻儿童中检测到解脲弯曲菌的首份报告。
