Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
PLoS One. 2012;7(11):e49188. doi: 10.1371/journal.pone.0049188. Epub 2012 Nov 19.
The bursa of Fabricius is critical for B cell development and differentiation in chick embryos. This study describes the production in vitro, from dissociated cell suspensions, of cellular agglomerates with functional similarities to the chicken bursa. Co-cultivation of epithelial and lymphoid cells obtained from embryos at the appropriate developmental stage regularly led to agglomerate formation within 48 hours. These agglomerates resembled bursal tissue in having lymphoid clusters overlaid by well organized epithelium. Whereas lymphocytes within agglomerates were predominantly Bu-1a(+), a majority of those emigrating onto the supporting membrane were Bu-1a(-) and IgM(+). Both agglomerates and emigrant cells expressed activation-induced deaminase with levels increasing after 24 hours. Emigrating cells were actively proliferating at a rate in excess of both the starting cell population and the population of cells remaining in agglomerates. The potential usefulness of this system for investigating the response of bursal tissue to avian Newcastle disease virus (strain AF2240) was examined.
法氏囊对于鸡胚 B 细胞的发育和分化至关重要。本研究描述了从分离的细胞悬液中体外产生具有类似于鸡法氏囊的功能的细胞聚集体。将来自适当发育阶段胚胎的上皮细胞和淋巴细胞共培养，通常会在 48 小时内形成聚集体。这些聚集体在具有被组织良好的上皮细胞覆盖的淋巴样簇的情况下类似于法氏囊组织。虽然聚集体内的淋巴细胞主要是 Bu-1a(+)，但大多数迁移到支持膜上的细胞是 Bu-1a(-)和 IgM(+)。聚集体和迁移细胞均表达激活诱导的脱氨酶，在 24 小时后水平增加。迁移细胞的增殖速度超过了起始细胞群体和聚集体中剩余细胞的群体。该系统对于研究法氏囊组织对禽新城疫病毒（AF2240 株）的反应具有潜在的用途。
