Autonomous University State of Mexico, Toluca, Mexico.
Aust Dent J. 2012 Dec;57(4):464-9. doi: 10.1111/adj.12004. Epub 2012 Oct 14.
The aim of this study was to quantitatively determine the adherence of Streptococcus mutans to orthodontic band cements.
Two hundred and ten blocks of seven different band cements for orthodontic prescription were made using a Teflon mould (4 × 4 × 1 mm). The obtained blocks were slightly polished and cleansed ultrasonically. Certified S. mutans ATCC 25175 were cultured with conventional methods for growth in Petri dishes and trypticase soy broth. Quantitative analysis was carried out with radioactive markers to codify the bacteria ((3) H). Subsequently, a combustion system was used to capture the residues, the radioactivity of the samples was measured, and the values recorded in disintegrations per minute (dpm). One-way analysis of variance (ANOVA) with a Scheffé test for multiple comparisons was realized with a significance level of ≤0.05.
Significant differences were found among different band cement materials (p < 0.001). Two band cement materials showed statistically lower values than the others (Transbond Plus Band Cem and Ketac Cem). In contrast, GC Fuji Ortho Band presented the highest adherence of S. mutans.
Among the cements evaluated, Transbond Plus Band Cem and Ketac Cem showed lower adherence of S. mutans.
本研究的目的是定量测定变异链球菌对正畸带粘结剂的黏附性。
使用聚四氟乙烯模具（4×4×1mm）制作 210 个七种不同正畸带粘结剂的块。获得的块体被轻微抛光并通过超声清洗。采用常规方法在培养皿和胰蛋白酶大豆肉汤中培养经认证的变异链球菌 ATCC 25175。用放射性标记物进行定量分析以对细菌进行编码((3)H)。随后，使用燃烧系统收集残留物，测量样品的放射性，并记录每分钟衰变数（dpm）。采用单因素方差分析（ANOVA）和Scheffé检验进行多重比较，显著性水平为≤0.05。
不同带粘结剂材料之间存在显著差异（p<0.001）。两种带粘结剂材料的数值明显低于其他材料（Transbond Plus Band Cem 和 Ketac Cem）。相比之下，GC Fuji Ortho Band 表现出最高的变异链球菌黏附性。
在所评估的粘结剂中，Transbond Plus Band Cem 和 Ketac Cem 显示出较低的变异链球菌黏附性。
