一种用于聚合酶链反应的DNA或RNA提取新方法。
A new method for extracting DNA or RNA for polymerase chain reaction.
作者信息
Yamada O, Matsumoto T, Nakashima M, Hagari S, Kamahora T, Ueyama H, Kishi Y, Uemura H, Kurimura T
机构信息
Department of Virology, Tottori University School of Medicine, Yonago, Japan.
出版信息
J Virol Methods. 1990 Feb;27(2):203-9. doi: 10.1016/0166-0934(90)90136-4.
The use of glass powder suspension for the extraction of RNA or DNA was studied to simplify the procedures of polymerase chain reaction (PCR). Using this procedure, proviral DNA of human T-lymphotropic virus type-1 (HTLV-1) in the blood of an asymptomatic virus carrier and viral RNA of human immunodeficiency virus (HIV) in the blood of an AIDS patient were easily detected by PCR employing glass powder. The use of glass powder is a simple and highly efficient procedure for the extraction of DNA or RNA, and can be applied for routine PCR.
研究了使用玻璃粉悬液提取RNA或DNA以简化聚合酶链反应（PCR）程序。采用该程序，通过使用玻璃粉的PCR，可轻松检测无症状病毒携带者血液中人类嗜T淋巴细胞病毒1型（HTLV-1）的前病毒DNA以及艾滋病患者血液中人类免疫缺陷病毒（HIV）的病毒RNA。使用玻璃粉是一种简单且高效的DNA或RNA提取程序，可应用于常规PCR。
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