Hill R J, Warren M K, Levin J
Department of Laboratory Medicine, University of California School of Medicine, San Francisco.
J Clin Invest. 1990 Apr;85(4):1242-7. doi: 10.1172/JCI114559.
To date, testing of various cytokines for the stimulation of blood cell production has not demonstrated a consistent effect on peripheral platelet levels. In this report, we provide evidence that human recombinant IL-6 increased platelet production in mice, as measured by both peripheral platelet levels and [75Se]selenomethionine (75SeM) incorporation into newly forming platelets. Peripheral white blood cell counts also were increased, but only to a modest extent, and hematocrit values were unchanged. A dose-response relationship between the amount of IL-6 administered and platelet count, 75SeM incorporation, and white blood cell count was demonstrated. Detectable megakaryocyte and granulocyte-macrophage colony-forming cells in mice that had received IL-6 also were increased in both bone marrow and spleen. These results demonstrate the ability of a purified, recombinant protein to stimulate platelet production in vivo.
迄今为止，对各种细胞因子刺激血细胞生成的测试尚未显示对外周血小板水平有一致的影响。在本报告中，我们提供证据表明，通过外周血小板水平和新形成血小板中[75硒]硒代蛋氨酸（75SeM）掺入量测量，重组人白细胞介素-6（IL-6）可增加小鼠血小板生成。外周白细胞计数也有所增加，但仅为适度增加，血细胞比容值未改变。显示了给予的IL-6量与血小板计数、75SeM掺入量和白细胞计数之间的剂量反应关系。接受IL-6的小鼠骨髓和脾脏中可检测到的巨核细胞以及粒细胞-巨噬细胞集落形成细胞也增加。这些结果证明了一种纯化的重组蛋白在体内刺激血小板生成的能力。
