Department of Dermatology, Universitat Autònoma de Barcelona, Barcelona, Spain.
J Invest Dermatol. 2013 Apr;133(4):999-1007. doi: 10.1038/jid.2012.418. Epub 2012 Nov 29.
Streptococcal throat infection is associated with a specific variant of psoriasis and with HLA-Cw6 expression. In this study, activation of circulating psoriatic cutaneous lymphocyte-associated antigen (CLA)(+) memory T cells cultured together with epidermal cells occurred only when streptococcal throat extracts were added. This triggered the production of Th1, Th17, and Th22 cytokines, as well as epidermal cell mediators (CXCL8, CXCL9, CXCL10, and CXCL11). Streptococcal extracts (SEs) did not induce any activation with either CLA(-) cells or memory T cells cultured together with epidermal cells from healthy subjects. Intradermal injection of activated culture supernatants into mouse skin induced epidermal hyperplasia. SEs also induced activation when we used epidermal cells from nonlesional skin of psoriatic patients with CLA(+) memory T cells. Significant correlations were found between SE induced upregulation of mRNA expression for ifn-γ, il-17, il-22, ip-10, and serum level of antistreptolysin O in psoriatic patients. This study demonstrates the direct involvement of streptococcal infection in pathological mechanisms of psoriasis, such as IL-17 production and epidermal cell activation.
链球菌性咽炎与特定类型的银屑病和 HLA-Cw6 表达相关。在本研究中，当加入链球菌咽提取物时，与表皮细胞共培养的循环银屑病皮肤淋巴细胞相关抗原（CLA）(+)记忆 T 细胞才会被激活。这会触发 Th1、Th17 和 Th22 细胞因子以及表皮细胞介质（CXCL8、CXCL9、CXCL10 和 CXCL11）的产生。链球菌提取物（SEs）不会诱导 CLA(-)细胞或与来自健康受试者的表皮细胞共培养的记忆 T 细胞发生任何激活。将激活的培养上清液皮内注射到小鼠皮肤中会诱导表皮增生。当我们使用来自银屑病患者非病变皮肤的表皮细胞和 CLA(+)记忆 T 细胞时，SE 也会诱导激活。在银屑病患者中，发现 SE 诱导的 ifn-γ、il-17、il-22、ip-10 和抗链球菌溶血素 O 血清水平的 mRNA 表达上调之间存在显著相关性。本研究表明链球菌感染直接参与了银屑病的病理机制，如 IL-17 的产生和表皮细胞的激活。
