Youn Hyung-Seop, Kim Mun-Kyoung, Kang Gil Bu, Kim Tae Gyun, An Jun Yop, Lee Jung-Gyu, Park Kyoung Ryoung, Lee Youngjin, Fukuoka Shin-Ichi, Eom Soo Hyun
School of Life Sciences, Cell Dynamics Research Center, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1488-90. doi: 10.1107/S1744309112040638. Epub 2012 Nov 14.
Quinolinate phosphoribosyltransferase (QAPRTase) is a key enzyme in NAD biosynthesis; it catalyzes the formation of nicotinate mononucleotide (NAMN) from quinolinate and 5-phosphoribosyl-1-pyrophosphate. In order to elucidate the mechanism of NAMN biosynthesis, crystals of Sus scrofa QAPRTase (Ss-QAPRTase) purified from porcine kidney in complex with NAMN were obtained and diffraction data were collected and processed to 2.1 Å resolution. The Ss-QAPRTase-NAMN cocrystals belonged to space group P321, with unit-cell parameters a=119.1, b=119.1, c=93.7 Å, γ=120.0°. The Matthews coefficient and the solvent content were estimated as 3.10 Å3 Da(-1) and 60.3%, respectively, assuming the presence of two molecules in the asymmetric unit.
喹啉酸磷酸核糖基转移酶（QAPRTase）是烟酰胺腺嘌呤二核苷酸（NAD）生物合成中的关键酶；它催化由喹啉酸和5-磷酸核糖-1-焦磷酸形成烟酸单核苷酸（NAMN）。为了阐明NAMN生物合成的机制，获得了从猪肾中纯化的猪QAPRTase（Ss-QAPRTase）与NAMN复合物的晶体，并收集了衍射数据，将其处理至2.1 Å分辨率。Ss-QAPRTase-NAMN共晶体属于P321空间群，晶胞参数为a = 119.1、b = 119.1、c = 93.7 Å，γ = 120.0°。假设不对称单元中存在两个分子，马修斯系数和溶剂含量分别估计为3.10 Å3 Da-1和60.3%。
