Moore D, Bice T, Jin L, Grandhi A, DeLucas L J, Narayana S V
Center for Macromolecular Crystallography, University of Alabama at Birmingham, AL 35294, USA.
Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):119-20. doi: 10.1107/s090744499700807x.
Quinolinate phosphoribosyltransferase (QPRT), purified from hog liver, has been crystallized using PEG 8000 as the precipitant. The crystals form long hexagonal rods in the space group P6322 with cell dimensions a = b = 121.7, c = 94.5 A. Based on the unit-cell dimensions and the calculated molecular mass of 33 500 Da, the Matthews coefficient suggests one molecule per asymmetric unit (Vm = 3.45 A3 Da-1; 64% solvent). Three native data sets were collected to a resolution of 2.5 A and merged to provide a set that is 94.7% complete, with an Rsym value of 9.6%.
