来自马氏甲烷八叠球菌的甲羟戊酸激酶的结晶及初步X射线衍射分析。
Crystallization and preliminary X-ray diffraction analysis of mevalonate kinase from Methanosarcina mazei.
作者信息
Zhuang Ningning, Seo Kyung Hye, Chen Cong, Zhou Jia, Kim Seon Won, Lee Kon Ho
机构信息
Division of Applied Life Science (BK21 Program), Gyeongsang National University, Jinju 660-701, Republic of Korea.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1560-3. doi: 10.1107/S1744309112047070. Epub 2012 Nov 28.
Abstract
Mevalonate kinase (MVK), which plays an important role in catalysing the biosynthesis of isoprenoid compounds derived from the mevalonate pathway, transforms mevalonate to 5-phosphomevalonate using ATP as a cofactor. Mevalonate kinase from Methanosarcina mazei (MmMVK) was expressed in Escherichia coli, purified and crystallized for structural analysis. Diffraction-quality crystals of MmMVK were obtained by the vapour-diffusion method using 0.32 M MgCl2, 0.08 M bis-tris pH 5.5, 16%(w/v) PEG 3350. The crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a=97.11, b=135.92, c=46.03 Å. Diffraction data were collected to 2.08 Å resolution.
摘要
甲羟戊酸激酶（MVK）在催化由甲羟戊酸途径衍生的类异戊二烯化合物的生物合成中起重要作用，它以ATP作为辅助因子将甲羟戊酸转化为5-磷酸甲羟戊酸。来自马氏甲烷八叠球菌（MmMVK）的甲羟戊酸激酶在大肠杆菌中表达、纯化并结晶以进行结构分析。通过气相扩散法，使用0.32 M MgCl2、0.08 M 双三羟甲基氨基甲烷pH 5.5、16%（w/v）聚乙二醇3350获得了MmMVK的衍射质量晶体。这些晶体属于空间群P2(1)2(1)2，晶胞参数a = 97.11、b = 135.92、c = 46.03 Å。衍射数据收集至2.08 Å分辨率。
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