鸢尾属（鸢尾科）中多态微卫星的分离与鉴定。
Isolation and characterization of polymorphic microsatellites in Iris ensata (Iridaceae).
机构信息
School of Resources and Environmental Sciences, East China Normal University, Shanghai 200062, People's Republic of China.
出版信息
Am J Bot. 2012 Dec;99(12):e498-500. doi: 10.3732/ajb.1200266. Epub 2012 Nov 28.
PREMISE OF THE STUDY
Microsatellite primers were developed in Iris ensata (Iridaceae) to provide polymorphic markers for further studies into population genetics. •
METHODS AND RESULTS
Thirteen polymorphic microsatellite loci were isolated from I. ensata. These loci were successfully amplified in two natural populations of I. ensata from eastern China (Longwangshan, Zhejiang Province) and northeastern China (Jinchuan, Jilin Province). There was no significant linkage disequilibrium found for any pair of loci. These loci contained between two and 12 alleles per locus across all 48 individuals of I. ensata. The number of alleles per locus varied from two to 10 at the population level and the observed and expected heterozygosities ranged from 0.167 to 0.958 and from 0.284 to 0.853, respectively. •
CONCLUSIONS
These loci showed high levels of polymorphism and could be used to study the population genetic structure, genetic relationships, and phylogeography of I. ensata.
研究前提
在鸢尾属（鸢尾科）中开发微卫星引物，为进一步研究种群遗传学提供多态性标记。
方法和结果
从中国东部（浙江龙王山）和中国东北部（吉林金川）的两个自然种群的鸢尾属中分离出 13 个多态性微卫星位点。这些位点在中国东部（浙江龙王山）和中国东北部（吉林金川）的两个自然种群的鸢尾属中成功扩增。任何一对位点之间均未发现显著的连锁不平衡。这些位点在 48 个鸢尾属个体中共包含 2 到 12 个等位基因。每个位点的等位基因数在种群水平上从 2 到 10 不等，观察到的和预期的杂合度分别在 0.167 到 0.958 之间和 0.284 到 0.853 之间。
结论
这些位点显示出高水平的多态性，可用于研究鸢尾属的种群遗传结构、遗传关系和系统地理学。
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