Homsy J, Meyer M, Levy J A
Department of Medicine, School of Medicine, University of California, San Francisco 94143-0128.
J Virol. 1990 Apr;64(4):1437-40. doi: 10.1128/JVI.64.4.1437-1440.1990.
The sera from 16 individuals infected with the human immunodeficiency virus (HIV) at different clinical stages were evaluated for antibody-dependent neutralization and/or enhancement of infectivity by HIV. The HIV isolate from each individual (homotypic) and established laboratory strains showing broad cellular host range and cytopathicity were used. All sera could neutralize one of the laboratory-passaged isolates, whereas only two could neutralize the corresponding homotypic strain. Seven homotypic isolates were enhanced by serum from the respective individual. This activity was primarily observed in patients with acquired immune deficiency syndrome. Moreover, the tropism for macrophages of four of these seven viral isolates was found to be enhanced by the homotypic sera. Finally, sequential pairs of HIV and sera obtained from five HIV-infected individuals with different clinical progression were studied over time. The enhancing activity of three of the five sera appeared to increase over time, indicating changes in both the host virus population and the type of antibodies produced. These results suggest that enhancing antibodies contribute to the spread and pathogenesis of HIV in vivo. They emphasize the necessity of studying further the association of enhancing antibodies and disease progression in infected individuals.
对16名处于不同临床阶段的人类免疫缺陷病毒（HIV）感染者的血清进行了评估，以检测其对HIV的抗体依赖性中和作用和/或感染性增强作用。使用了来自每个个体的HIV分离株（同型）以及具有广泛细胞宿主范围和细胞病变效应的既定实验室菌株。所有血清都能中和一种实验室传代分离株，而只有两份血清能中和相应的同型菌株。七种同型分离株被各自个体的血清增强了感染性。这种活性主要在获得性免疫缺陷综合征患者中观察到。此外，发现这七种病毒分离株中的四种对巨噬细胞的嗜性被同型血清增强。最后，对五名具有不同临床进展的HIV感染者随时间获取的HIV和血清序列对进行了研究。五份血清中的三份的增强活性似乎随时间增加，表明宿主病毒群体和产生的抗体类型都发生了变化。这些结果表明，增强性抗体在体内促进了HIV的传播和发病机制。它们强调了进一步研究感染个体中增强性抗体与疾病进展之间关联的必要性。
