Böttcher-Haberzeth Sophie, Klar Agnieszka S, Biedermann Thomas, Schiestl Clemens, Meuli-Simmen Claudia, Reichmann Ernst, Meuli Martin
Tissue Biology Research Unit, University Children's Hospital Zurich, Zurich, Switzerland.
Pediatr Surg Int. 2013 Mar;29(3):239-47. doi: 10.1007/s00383-012-3217-0. Epub 2012 Nov 30.
Autologous skin substitutes to cover large skin defects are used since several years. Melanocytes, although essential for solar protection and pigmentation of skin, are not yet systematically added to such substitutes. In this experimental study, we reconstructed melanocyte-containing dermo-epidermal skin substitutes from donor skins of different skin pigmentation types and studied them in an animal model. Features pertinent to skin color were analyzed and compared in both skin substitutes and original donor skin.
Keratinocytes, melanocytes, and fibroblast were isolated, cultured, and expanded from skin biopsies of light- and dark-pigmented patients. For each donor, melanocytes and keratinocytes were seeded in different ratios (1:1, 1:5, 1:10) onto collagen gels previously populated with autologous fibroblasts. Skin substitutes were then transplanted onto full-thickness wounds of immuno-incompetent rats. After 8 weeks, macroscopic and microscopic analyses were conducted with regard to skin color and architecture.
Chromameter evaluation revealed that skin color of reconstructed light- and dark-pigmented skin was very similar to donor skin, independent of which melanocyte/keratinocyte ratio was added. Histological analyses of the skin analogs confirmed these findings.
These data suggest that adding autologous melanocytes to bioengineered dermo-epidermal skin analogs can sustainably restore the patients' native skin color.
多年来一直使用自体皮肤替代物来覆盖大面积皮肤缺损。黑素细胞虽然对皮肤的防晒和色素沉着至关重要，但尚未被系统地添加到此类替代物中。在本实验研究中，我们从不同皮肤色素沉着类型的供体皮肤中构建了含黑素细胞的真皮 - 表皮皮肤替代物，并在动物模型中对其进行研究。对皮肤替代物和原始供体皮肤中与肤色相关的特征进行了分析和比较。
从浅色和深色色素沉着患者的皮肤活检组织中分离、培养和扩增角质形成细胞、黑素细胞和成纤维细胞。对于每个供体，将黑素细胞和角质形成细胞以不同比例（1:1、1:5、1:10）接种到预先接种有自体成纤维细胞的胶原凝胶上。然后将皮肤替代物移植到免疫缺陷大鼠的全层伤口上。8周后，对皮肤颜色和结构进行宏观和微观分析。
色差仪评估显示，重建的浅色和深色色素沉着皮肤的颜色与供体皮肤非常相似，与添加的黑素细胞/角质形成细胞比例无关。皮肤类似物的组织学分析证实了这些发现。
这些数据表明，将自体黑素细胞添加到生物工程真皮 - 表皮皮肤类似物中可以可持续地恢复患者的天然肤色。
