Barmettler Gery, Ziegler Urs
Center for Microscopy and Image Analysis, University of Zurich, Zurich, Switzerland.
Methods Mol Biol. 2025;2922:229-240. doi: 10.1007/978-1-0716-4510-9_17.
This chapter is intended to be a guide to skin and skin substitutes studies dealing with the analysis of subcellular structures of cells and tissues by providing an exhaustive sample preparation protocol for Transmission Electron Microscopy (TEM). Critical steps to obtain good ultrastructure are mentioned as well as notes to improve the sample collection. New ways to automatize the imaging acquisition are discussed.
本章旨在通过提供详尽的透射电子显微镜（TEM）样品制备方案，为涉及细胞和组织亚细胞结构分析的皮肤及皮肤替代物研究提供指导。文中提到了获得良好超微结构的关键步骤以及改进样品采集的注意事项。还讨论了自动成像采集的新方法。
