Paajanen H, Reisto T, Hemmilä I, Komu M, Niemi P, Kormano M
Department of Diagnostic Radiology, University of Turku, Finland.
Magn Reson Med. 1990 Jan;13(1):38-43. doi: 10.1002/mrm.1910130106.
Bovine serum albumin, immunoglobulin G, and fibrinogen were labeled with Gd-DTPA using a bifunctional chelating agent DTPA anhydride. The protein-(Gd-DTPA) conjugates had 1.4- to 2.0-fold greater longitudinal relaxivities at 0.02 and 0.44 T than the relaxivity of plain Gd-DTPA. The increase of longitudinal relaxivity was not directly related to the size of carrier protein. Up to 50 Gd-DTPA chelates per protein, longitudinal relaxivity of the conjugates was proportional to the concentration of Gd and independent of the Gd/protein ratio.
使用双功能螯合剂二酐二乙三胺五乙酸（DTPA酐）将牛血清白蛋白、免疫球蛋白G和纤维蛋白原用钆-二乙三胺五乙酸（Gd-DTPA）进行标记。在0.02和0.44特斯拉的磁场强度下，蛋白质-（Gd-DTPA）缀合物的纵向弛豫率比普通Gd-DTPA的弛豫率高1.4至2.0倍。纵向弛豫率的增加与载体蛋白的大小没有直接关系。每个蛋白质上最多有50个Gd-DTPA螯合物时，缀合物的纵向弛豫率与Gd的浓度成正比，且与Gd/蛋白质比例无关。
