Department of Plant Ecophysiology, Faculty of Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland.
Plant Sci. 2013 Jan;198:83-90. doi: 10.1016/j.plantsci.2012.10.004. Epub 2012 Oct 23.
The primed mobilization for more potent defense responses to subsequent stress has been shown for many plant species, but there is a growing need to identify reliable molecular markers for this unique phenomenon. In the present study a proteomic approach was used to screen similarities in protein abundance in leaves of primed potato (Solanum tuberosum L.) treated with four well-known inducers of plant resistance, i.e. β-aminobutyric acid (BABA), γ-aminobutyric acid (GABA), Laminarin and 2,6-dichloroisonicotinic acid (INA), respectively. Moreover, to gain insight into the importance of nitric oxide (NO) in primed protein accumulation the potato leaves were supplied by S-nitrosoglutathione (GSNO), as an NO donor. The comparative analysis, using two-dimensional electrophoresis and mass spectrometry, revealed that among 25 proteins accumulated specifically after BABA, GABA, INA and Laminarin treatments, 13 proteins were accumulated also in response to GSNO. Additionally, overlapping proteomic changes between BABA-primed and GSNO-treated leaves showed 5 protein spots absent in the proteome maps obtained in response to the other priming agents. The identified 18 proteins belonged, in most cases, to functional categories of primary metabolism. The selected proteins including three redox-regulated enzymes, i.e. glyceraldehyde 3-phosphate dehydrogenase, carbonic anhydrase, and fructose-bisphosphate aldolase, were discussed in relation to the plant defence responses. Taken together, the overlapping effects in the protein profiles obtained between priming agents, GSNO and cPTIO treatments provide insight indicating that the primed potato exhibits unique changes in the primary metabolism, associated with selective protein modification via NO.
植物的许多种属都存在这种被预先处理激活，以产生对随后压力更强防御反应的现象，目前人们越来越需要识别这种独特现象的可靠分子标记物。在本研究中，采用蛋白质组学方法筛选经 4 种植物抗性诱导剂（即β-氨基丁酸（BABA）、γ-氨基丁酸（GABA）、海藻糖和 2,6-二氯异烟酸（INA））处理的预先处理马铃薯（Solanum tuberosum L.）叶片中蛋白质丰度的相似性。此外，为了深入了解一氧化氮（NO）在预先处理蛋白积累中的重要性，用 S-亚硝基谷胱甘肽（GSNO）作为 NO 供体处理马铃薯叶片。使用二维电泳和质谱的比较分析显示，在 BABA、GABA、INA 和海藻糖处理后特异性积累的 25 种蛋白质中，有 13 种蛋白质也响应 GSNO 而积累。此外，BABA 预先处理和 GSNO 处理叶片之间的蛋白质组学变化重叠显示，在响应其他预激剂的蛋白质图谱中没有 5 个蛋白质斑点。鉴定出的 18 种蛋白质大多属于初级代谢的功能类别。所选择的蛋白质包括三种氧化还原调节酶，即甘油醛 3-磷酸脱氢酶、碳酸酐酶和果糖二磷酸醛缩酶，与植物防御反应有关。总的来说，在预激剂、GSNO 和 cPTIO 处理之间获得的蛋白质图谱中重叠的效应表明，预先处理的马铃薯表现出与一氧化氮（NO）选择性蛋白修饰相关的初级代谢的独特变化。
