Departments of Pharmacognosy, Faculty of Pharmacy and Traditional Iranian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran.
Asian Pac J Trop Med. 2012 Dec;5(12):962-5. doi: 10.1016/S1995-7645(12)60182-5.
To investigate the larvicidal activity of essential oil and methanol extract of the Nepeta menthoides (N. menthoides) against main malaria vector, Anopheles stephensi (An. stephensi).
The essential oil of plant was obtained by Clevenger type apparatus and the methanol extract was supplied with Percolation method. Larvicidal activity was tested by WHO method. Twenty five fourth-instar larvae of An. stephensi were used in the larvicidal assay and four replicates were tested for each concentration. Five different concentrations of the oil and extract were tested for calculation of LC(50) and LC(90) values.
The LC(50) and LC(90) values were determined by probit analysis. LC(50) was 69.5 and 234.3 ppm and LC(90) was 175.5 and 419.9 ppm for the extract and essential oil respectively.
According to the results of this study methanolic extract of plant exhibited more larvicidal activity than essential oil. This could be useful for investigation of new natural larvicidal compounds.
研究荆芥（Nepeta menthoides）精油和甲醇提取物对主要疟疾传播媒介按蚊（Anopheles stephensi）的杀幼虫活性。
采用克利夫兰型仪器提取植物精油，采用渗滤法提供甲醇提取物。采用世界卫生组织（WHO）方法进行杀幼虫活性测试。在杀幼虫测定中使用 25 只第四龄按蚊幼虫，每个浓度测试 4 个重复。测试了五种不同浓度的油和提取物，以计算 LC（50）和 LC（90）值。
通过概率分析确定 LC（50）和 LC（90）值。提取物和精油的 LC（50）分别为 69.5 和 234.3 ppm，LC（90）分别为 175.5 和 419.9 ppm。
根据本研究的结果，植物甲醇提取物的杀幼虫活性比精油更强。这可能有助于研究新的天然杀幼虫化合物。
