辣根过氧化物酶。三十四。高碘酸盐和无机阴离子自由基将化合物II氧化为I 。

Horseradish peroxidase. XXXIV. Oxidation of compound II to I by periodate and inorganic anion radicals.

作者信息

Nadezhdin A, Dunford H B

出版信息

Can J Biochem. 1979 Aug;57(8):1080-3. doi: 10.1139/o79-137.

PMID:232001

Abstract

The one-electron oxidation of horseradish peroxidase compound II to compound I by sodium periodate was observed. The bimolecular rate constant for the NaIO4--compound II interaction is equal to 9.5 +/- 1 x 10(-3) M-1s-1 at room temperature. Irradiation, using ultraviolet light, of the solution containing compound II and persulfate in the presence of bicarbonate, chloride, or bromide, leads ot the fast accumulation of compound I due to the oxidative action of SO4, CO3, Cl2, and Br2 anion radicals, which are products of the photolysis.

摘要

观察到高碘酸钠将辣根过氧化物酶化合物II单电子氧化为化合物I的过程。在室温下，NaIO₄与化合物II相互作用的双分子速率常数等于9.5±1×10⁻³ M⁻¹s⁻¹。在碳酸氢根、氯离子或溴离子存在的情况下，用紫外光照射含有化合物II和过硫酸盐的溶液，由于光解产物SO₄、CO₃、Cl₂和Br₂阴离子自由基的氧化作用，会导致化合物I快速积累。