Klatka Janusz, Grywalska Ewelina, Wasiak Magdalena, Wdowiak Paulina, Andrzejczak Adrian, Trzaskowska Eliza, Roliński Jacek
Katedra i Klinika Otolaryngologii i Onkologii Laryngologicznej Uniwersytetu Medycznego w Lublinie, Poland.
Otolaryngol Pol. 2012 Nov-Dec;66(6):413-8. doi: 10.1016/j.otpol.2012.06.005. Epub 2012 Jun 9.
B7-H1, B7-H2 and B7-H4 molecules play various roles in the adaptive immune system and are broadly expressed on many cells, especially those localized in cancer tissue. The aim of the study was to assess the surface expression of B7-H1, B7-H2 and B7-H4 molecules on the mature dendritic cells (DC) generated from peripheral blood monocytes of laryngeal cancer patients and healthy donors.
Forty-four male patients treated surgically for squamous cell carcinoma of the larynx were included in the study. Peripheral blood from twelve healthy male donors was used as a control. Mononuclear cells were separated from all individuals by density gradient centrifugation, incubated with anti-CD14 microbeads, and passed through MACS separation columns. The CD14 positive cell population was used to prepare monocyte derived DC. Laryngeal cancer tissue was obtained from patients during surgical treatment and homogenized to prepare tumor cell lysates for further stimulation. We evaluated with the use of flow cytometry method the percentage of cells with an expression and mean fluorescent intensity (MFI) of surface markers, such as: CD83, B7-H1, B7-H2, B7-H4.
Our study revealed that the percentage of mature dendritic cells, stimulated with autologous tumor cell lysates, with the expression of B7-H1 molecule in patients was lower than in healthy donors (61.81 ± 25.58% vs 93.02 ± 4.63%, p=0.007). In laryngeal cancer patients, the percentage of CD83+/B7-H2+ cells was higher than in healthy individuals (18.32 ± 10.74% vs 2.89 ± 0.43%, p=0.019).
There is a relation between the presence of laryngeal cancer and the expression of B7 family molecules.
B7-H1、B7-H2和B7-H4分子在适应性免疫系统中发挥多种作用，并广泛表达于许多细胞上，尤其是那些位于癌组织中的细胞。本研究的目的是评估B7-H1、B7-H2和B7-H4分子在喉癌患者和健康供体外周血单核细胞生成的成熟树突状细胞（DC）上的表面表达。
本研究纳入了44例接受喉鳞状细胞癌手术治疗的男性患者。来自12名健康男性供体的外周血用作对照。通过密度梯度离心从所有个体中分离单核细胞，与抗CD14微珠孵育，然后通过MACS分离柱。CD14阳性细胞群体用于制备单核细胞衍生的DC。在手术治疗期间从患者处获取喉癌组织并匀浆以制备肿瘤细胞裂解物用于进一步刺激。我们使用流式细胞术方法评估了具有表面标志物表达的细胞百分比和平均荧光强度（MFI），这些标志物包括：CD83、B7-H1、B7-H2、B7-H4。
我们的研究表明，用自体肿瘤细胞裂解物刺激的成熟树突状细胞中，患者B7-H1分子表达的百分比低于健康供体（61.81±25.58%对93.02±4.63%，p = 0.007）。在喉癌患者中，CD83+/B7-H2+细胞的百分比高于健康个体（18.32±10.74%对2.89±0.43%，p = 0.019）。
喉癌的存在与B7家族分子的表达之间存在关联。
