Department of Otolaryngology and Laryngeal Oncology, Medical University of Lublin, Jaczewskiego 8 Street, 20-954 Lublin, Poland.
Eur Arch Otorhinolaryngol. 2013 Sep;270(10):2683-93. doi: 10.1007/s00405-013-2510-4. Epub 2013 Apr 30.
B7H1 and B7H4 overexpression is associated with inhibition of the immune system in many solid tumors, and altogether with CD200 molecule plays an important role in tumor invasion by promoting malignant transformation. However, there is no report about impact of these molecules on laryngeal squamous cell carcinoma. The objective of the present study was to assess by means of flow cytometry the expression of B7H1, B7H4, CD200, and CD200R on CD83+ monocyte-derived dendritic cells (Mo-DC), pulsed with autologous tumor cell lysates (aTCL) in patients who suffer from G1, G2, or G3 laryngeal carcinoma (LC, n = 60) in comparison to healthy donors (HD, n = 15). It has been demonstrated that median value of the percentages of CD83+ B7H1+, CD83+ B7H4+, and CD83+ CD200+ cells were higher in LC patients than HD (p = 0.041, p ≤ 0.0001, and p = 0.02, respectively). Mean fluorescence intensity (MFI) of CD200, CD200R, B7H1, and B7H4 on the Mo-DC pulsed with aTCL of the patients was also higher than on the Mo-DC of HD (p ≤ 0.0001, p ≤ 0.0001, p = 0.002, and p ≤ 0.0001, respectively). The highest MFI levels of all molecules were noted in grade 3 LC. The aforementioned results prove that there is a relation between the presence of laryngeal cancer and the expression of B7H1, B7H4, CD200, and CD200R regulatory molecules on the CD83+ Mo-DC pulsed with autologous cancer cell lysates. Strong association of LC grade and the tested antigens expression suggests a critical role for these proteins in LC biology.
B7H1 和 B7H4 的过度表达与许多实体瘤中免疫系统的抑制有关，而与 CD200 分子一起，通过促进恶性转化，在肿瘤侵袭中发挥重要作用。然而，目前还没有关于这些分子对喉鳞状细胞癌影响的报道。本研究的目的是通过流式细胞术评估 CD83+单核细胞衍生树突状细胞（Mo-DC）在与健康供体（HD，n=15）相比，来自患有 G1、G2 或 G3 喉癌（LC，n=60）患者的自体肿瘤细胞裂解物（aTCL）刺激后表达 B7H1、B7H4、CD200 和 CD200R 的情况。结果表明，LC 患者的 CD83+B7H1+、CD83+B7H4+和 CD83+CD200+细胞的中位数百分比高于 HD（p=0.041、p≤0.0001 和 p=0.02）。用 aTCL 刺激的 Mo-DC 上 CD200、CD200R、B7H1 和 B7H4 的平均荧光强度（MFI）也高于 HD 的 Mo-DC（p≤0.0001、p≤0.0001、p=0.002 和 p≤0.0001）。所有分子的最高 MFI 水平均见于 3 级 LC。上述结果证明，喉癌的存在与 CD83+Mo-DC 上表达的 B7H1、B7H4、CD200 和 CD200R 调节分子之间存在关联，这些分子用自体癌细胞裂解物刺激。LC 分级与所测试抗原表达之间的强关联表明这些蛋白质在 LC 生物学中起关键作用。
