Food and Biobased Research, Wageningen University and Research Centre, Bornse Weilanden 9, 6708 CT Wageningen, The Netherlands.
Bioresour Technol. 2013 Jan;128:431-7. doi: 10.1016/j.biortech.2012.10.094. Epub 2012 Nov 7.
Green seaweed Ulva lactuca harvested from the North Sea near Zeeland (The Netherlands) was characterized as feedstock for acetone, ethanol and ethanol fermentation. Solubilization of over 90% of sugars was achieved by hot-water treatment followed by hydrolysis using commercial cellulases. A hydrolysate was used for the production of acetone, butanol and ethanol (ABE) by Clostridium acetobutylicum and Clostridium beijerinckii. Hydrolysate-based media were fermentable without nutrient supplementation. C. beijerinckii utilized all sugars in the hydrolysate and produced ABE at high yields (0.35 g ABE/g sugar consumed), while C. acetobutylicum produced mostly organic acids (acetic and butyric acids). These results demonstrate the great potential of U. lactuca as feedstock for fermentation. Interestingly, in control cultures of C. beijerinckii on rhamnose and glucose, 1,2 propanediol was the main fermentation product (9.7 g/L).
从荷兰泽兰省附近的北海收获的绿藻 U. lactuca 被用作丙酮、乙醇和乙醇发酵的原料。热水处理后，用商业纤维素酶水解，超过 90%的糖得到溶解。用水解产物通过丙酮丁醇梭菌和拜氏梭菌生产丙酮、丁醇和乙醇（ABE）。基于水解产物的培养基无需营养补充即可发酵。C. beijerinckii 利用水解产物中的所有糖，并以高收率（0.35 g ABE/g 消耗的糖）生产 ABE，而 C. acetobutylicum 主要产生有机酸（乙酸和丁酸）。这些结果表明 U. lactuca 作为发酵原料具有巨大的潜力。有趣的是，在 C. beijerinckii 以鼠李糖和葡萄糖为对照培养物中，1,2-丙二醇是主要发酵产物（9.7 g/L）。
