CLIB Graduate Cluster Industrial Biotechnology, Centrum für Biotechnologie, Universität Bielefeld, 33615 Bielefeld, Germany.
J Biotechnol. 2013 Aug 20;167(2):135-41. doi: 10.1016/j.jbiotec.2012.11.003. Epub 2012 Nov 29.
Corynebacterium pseudotuberculosis is the causative agent of several veterinary diseases in a broad range of economically important hosts, which can vary from caseous lymphadenitis in sheep and goats (biovar ovis) to ulcerative lymphangitis in cattle and horses (biovar equi). Existing vaccines against C. pseudotuberculosis are mainly intended for small ruminants and, even in these hosts, they still present remarkable limitations. In this study, we present the complete genome sequence of C. pseudotuberculosis biovar equi strain 258, isolated from a horse with ulcerative lymphangitis. The genome has a total size of 2,314,404 bp and contains 2088 predicted protein-coding regions. Using in silico analysis, eleven pathogenicity islands were detected in the genome sequence of C. pseudotuberculosis 258. The application of a reverse vaccinology strategy identified 49 putative antigenic proteins, which can be used as candidate vaccine targets in future works.
类鼻疽伯克霍尔德菌是广泛的经济重要宿主的多种兽医疾病的病原体，这些宿主可从绵羊和山羊的干酪性淋巴结炎（生物型ovis）到牛和马的溃疡性淋巴管炎（生物型equi）不等。现有的针对类鼻疽伯克霍尔德菌的疫苗主要针对小反刍动物，即使在这些宿主中，它们仍然存在显著的局限性。在本研究中，我们介绍了从患有溃疡性淋巴管炎的马中分离到的类鼻疽伯克霍尔德菌生物型 equi 菌株 258 的完整基因组序列。该基因组的总大小为 2,314,404 bp，包含 2088 个预测的蛋白编码区。通过计算机分析，在类鼻疽伯克霍尔德菌 258 的基因组序列中检测到了 11 个致病性岛。反向疫苗学策略的应用鉴定了 49 种潜在的抗原蛋白，这些蛋白可作为未来工作中的候选疫苗靶标。
