新型芘-苝二聚体 2'-α-L-氨基-LNA FRET 对稳定和监测分支 DNA 纳米结构的高效作用。
Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene-perylene 2'-α-L-amino-LNA FRET pairs.
机构信息
Nucleic Acid Center, Department of Physics, Chemistry and Pharmacy, University of Southern Denmark, DK-5230 Odense M, Denmark.
出版信息
Chem Commun (Camb). 2013 Jan 18;49(5):511-3. doi: 10.1039/c2cc37547h.
Novel pyrene-perylene α-L-LNA FRET pairs described herein effectively detect assembly of 2- and 3-way branched DNA nanostructures prepared by postsynthetic microwave-assisted CuAAC click chemistry. The fluorescent signalling of assembly by internally positioned FRET pairs is achieved with low to no fluorescence background signal, remarkably low limit of target detection values and stabilization of the resulting nanostructures.
本文所述的新型苝-并苯 α-L-LNA FRET 对有效地检测了通过后合成微波辅助 CuAAC 点击化学制备的 2 路和 3 路分支 DNA 纳米结构的组装。通过内部定位的 FRET 对进行组装的荧光信号实现了低至无荧光背景信号、显著低的目标检测值下限和所得纳米结构的稳定化。
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