García-Lorenzo Andrés, Rodríguez-Piñeiro Ana M, Rodríguez-Berrocal Francisco J, Cadena María Páez de la, Martínez-Zorzano Vicenta S
Department of Biochemistry, Genetics and Immunology, Faculty of Biology, University of Vigo, 36310 Vigo, Spain.
Int J Mol Sci. 2012 Nov 7;13(11):14401-20. doi: 10.3390/ijms131114401.
Colorectal cancer is still a major health burden worldwide, and its diagnosis has not improved in recent years due to a lack of appropriate diagnostic serum markers. Aiming to find new diagnostic proteins, we applied the proteomic DIGE technology to analyze changes in the secretome before/after differentiation of the colon adenocarcinoma Caco-2 cell line, an accepted in vitro model to study colorectal tumorigenesis. When the secretomes from undifferentiated (tumor-like) and differentiated cells (resembling healthy enterocytes) were compared, we found 96 spots differentially expressed. After MS/MS analysis, 22 spots corresponding to 15 different proteins were identified. Principal component analysis demonstrated these 22 spots could serve as a discriminatory panel between the tumor-like and normal-like cells. Among the identified proteins, the translationally-controlled tumor protein (TCTP), the transforming growth factor-beta-induced protein ig-h3 (TGFβIp), and the Niemann-Pick disease type C2 protein (NPC2) are interesting candidates for future studies focused on their utility as serum biomarkers of colorectal cancer.
结直肠癌仍是全球主要的健康负担，由于缺乏合适的诊断血清标志物，近年来其诊断水平并未得到改善。为了寻找新的诊断蛋白，我们应用蛋白质组双向荧光差异凝胶电泳技术分析结肠腺癌Caco-2细胞系分化前后分泌蛋白质组的变化，Caco-2细胞系是研究结直肠癌发生的公认体外模型。当比较未分化（肿瘤样）细胞和分化细胞（类似健康肠上皮细胞）的分泌蛋白质组时，我们发现96个差异表达的斑点。经过串联质谱分析，鉴定出对应于15种不同蛋白质的22个斑点。主成分分析表明，这22个斑点可作为区分肿瘤样细胞和正常样细胞的判别组。在鉴定出的蛋白质中，翻译控制肿瘤蛋白（TCTP）、转化生长因子-β诱导蛋白ig-h3（TGFβIp）和尼曼-匹克病C2型蛋白（NPC2）是未来研究的有趣候选物，这些研究聚焦于它们作为结直肠癌血清生物标志物的效用。
