Zheng H J, Tao Z H, Reddy M V, Harinath B C, Piessens W F
Am J Trop Med Hyg. 1987 May;36(3):554-60. doi: 10.4269/ajtmh.1987.36.554.
We developed a sandwich enzyme-linked immunosorbent assay to detect circulating parasite antigens in human lymphatic filariasis. The assay utilizes a polyclonal rabbit antifilarial antiserum to capture, and a monoclonal antibody to identify, circulating parasite antigen. Using this assay, we found that greater than 95% of sera from microfilaremic donors with bancroftian or brugian filariasis, approximately 60% of sera from microfilaremic patients with hydroceles, chyluria, or elephantiasis, and 15%-20% of sera from asymptomatic residents of filariasis-endemic areas evidently contain filarial antigens. Antigen was also detected in the urine of some microfilaremic patients. Serum levels of antigen detected with one monoclonal antibody, ES34, correlated well with microfilarial density in night blood. In contrast, less than 5% of sera from residents of areas where lymphatic filariasis is not endemic reacted in the assay, even though approximately one-third of the donors whose sera were tested were known to be infected with intestinal nematodes. The assay was designed to be flexible enough to allow the parallel use of multiple monoclonal antibodies with different specificities and simple enough to be applicable in most areas where lymphatic filariasis is endemic.
我们开发了一种夹心酶联免疫吸附测定法来检测人类淋巴丝虫病患者血液中的循环寄生虫抗原。该测定法利用兔抗丝虫多克隆抗血清进行捕获，并使用单克隆抗体来识别循环寄生虫抗原。通过该测定法，我们发现，超过95%的班氏或布鲁氏丝虫病微丝蚴血症患者的血清、约60%的患有鞘膜积液、乳糜尿或象皮肿的微丝蚴血症患者的血清，以及15%-20%的丝虫病流行地区无症状居民的血清中明显含有丝虫抗原。在一些微丝蚴血症患者的尿液中也检测到了抗原。用一种单克隆抗体ES34检测到的血清抗原水平与夜间血液中的微丝蚴密度密切相关。相比之下，在淋巴丝虫病非流行地区居民的血清中，只有不到5%的血清在该测定法中出现反应，尽管接受血清检测的捐赠者中约有三分之一已知感染了肠道线虫。该测定法设计得足够灵活，允许同时使用多种具有不同特异性的单克隆抗体，并且足够简单，适用于大多数淋巴丝虫病流行地区。
