Horiuchi M, Takase K, Nomura M, Chiba T
Nihon Yakurigaku Zasshi. 1979 Jul;75(5):433-45.
Effects of oral administration of tiopronin (2-mercaptopropionylglycine) on acute liver damage induced in rats by intraperitoneal administration of carbon tetrachloride (CCl4) were investigated. Tiopronin suppressed increase in serum transaminase activity, accumulation of liver triglyceride and decrease of liver glucose-6-phosphatase activity induced by CCl4. CCl4 induced a significant decrease of nonprotein thiol (NPSH) in the liver 24 hr after administration, but this decrease did not result in an increase of nonprotein disulfide in the liver. Tiopronin suppressed the decrease in NPSH induced by CCl4, but did not influence NPSH in normal rats 24 hr after administration. In addition to these biochemical findings, it was also noted that tiopronin prevented necrosis and decrease of glycogen in liver, as determined histologically. Other compounds such as cystamine, cysteine and glutathione proved to have a preventive effect on CCl4-induced liver damage as in the case of tiopronin. It was revealed that such preventive effect correlated to some extent with NPSH content in liver as well as serum transaminase activity and histological findings.
研究了口服硫普罗宁（2-巯基丙酰甘氨酸）对腹腔注射四氯化碳（CCl4）诱导的大鼠急性肝损伤的影响。硫普罗宁可抑制CCl4诱导的血清转氨酶活性升高、肝脏甘油三酯蓄积以及肝脏葡萄糖-6-磷酸酶活性降低。给药24小时后，CCl4可导致肝脏中非蛋白硫醇（NPSH）显著降低，但这种降低并未导致肝脏中非蛋白二硫键增加。硫普罗宁可抑制CCl4诱导的NPSH降低，但对正常大鼠给药24小时后的NPSH无影响。除了这些生化结果外，组织学检查还发现硫普罗宁可预防肝脏坏死和糖原减少。与硫普罗宁一样，胱胺、半胱氨酸和谷胱甘肽等其他化合物对CCl4诱导的肝损伤也有预防作用。结果表明，这种预防作用在一定程度上与肝脏中的NPSH含量、血清转氨酶活性以及组织学结果相关。
