Chiba T, Horiuchi M, Akashi Y
Nihon Yakurigaku Zasshi. 1979 Sep;75(6):551-62.
Preventive and therapeutic effects of tiopronin (2-mercaptopropionylglycine) and glutathione on ethionine induced liver damage were studied. Administration of 1 g/kg ethionine resulted in significant differences in the degree of liver damage, and such was dependent on the feeding conditions of the animals. The present experiment was performed under the conditions where the most serious liver damage was observed. In the experiment on the preventive effects, serum GOT and GPT were markedly elevated by ethionine, but such elevation could be suppressed by administering tiopronin or glutathione 10 min before ethionine administration. Liver nonprotein thiol (NPSH) content decreased by 40--60% of the normal level 16 hr after ethionine adminstration, but increased by 30--45% 24 hr later. Administration of tiopronin suppressed the initial fall of liver NPSH content caused by ethionine, but this tendency was not observed in the glutathione treatment. Both liver cholesterol and triglyceride increased in the ethionine treated rats, and triglycerides in particular decreased with administration of tiopronin or glutathione. In the experiment on the therapeutic effects, the maximal values of serum GOT and GPT brought by ethionine were suppressed by the thiol compounds given 16 hr after ethionine administration, but liver NPSH content and liver lipids were not influenced. Thus, tiopronin and glutathione are considered to have preventive and therapeutic effects on liver damage induced by ethionine.
研究了硫普罗宁（2-巯基丙酰甘氨酸）和谷胱甘肽对乙硫氨酸诱导的肝损伤的预防和治疗作用。给予1g/kg乙硫氨酸会导致肝损伤程度出现显著差异，且这种差异取决于动物的饲养条件。本实验是在观察到最严重肝损伤的条件下进行的。在预防作用实验中，乙硫氨酸使血清谷草转氨酶（GOT）和谷丙转氨酶（GPT）显著升高，但在给予乙硫氨酸前10分钟给予硫普罗宁或谷胱甘肽可抑制这种升高。乙硫氨酸给药16小时后，肝脏非蛋白巯基（NPSH）含量降至正常水平的40%-60%，但24小时后升高30%-45%。给予硫普罗宁可抑制乙硫氨酸引起的肝脏NPSH含量的初始下降，但谷胱甘肽治疗未观察到这种趋势。乙硫氨酸处理的大鼠肝脏胆固醇和甘油三酯均升高，特别是甘油三酯在给予硫普罗宁或谷胱甘肽后降低。在治疗作用实验中，乙硫氨酸给药16小时后给予硫醇化合物可抑制乙硫氨酸引起的血清GOT和GPT的最大值，但肝脏NPSH含量和肝脏脂质未受影响。因此，硫普罗宁和谷胱甘肽被认为对乙硫氨酸诱导的肝损伤具有预防和治疗作用。
