Dairy and Swine Research and Development Centre, Agriculture and Agri-Food Canada, 2000 College, Sherbrooke, Quebec J1M 0C8, Canada.
Water Res. 2013 Feb 1;47(2):737-46. doi: 10.1016/j.watres.2012.10.047. Epub 2012 Nov 12.
Greenhouse gas emissions represent a major environmental problem associated with the management of manure from the livestock industry. Methane is the primary GHG emitted during manure outdoor storage. In this paper, the variability of two swine and two dairy manure storage tanks was surveyed, in terms of physico-chemical and microbiological parameters. The impact of the inter-tank and spatio-temporal variations of these parameters on the methanogenic activity of manure was ascertained. A Partial Least Square regression was carried out, which demonstrated that physico-chemical as well as microbiological parameters had a major influence on the methanogenic activity. Among the 19 parameters included in the regression, the concentrations of VFAs had the strongest negative influence on the methane emission rate of manure, resulting from their well-known inhibitory effect. The relative abundance of two amplicons in archaeal fingerprints was found to positively influence the methanogenic activity, suggesting that Methanoculleus spp. and possibly Methanosarcina spp. are major contributors to methanogenesis in storage tanks. This work gave insights into the mechanisms, which drive methanogenesis in swine and dairy manure storage tanks.
温室气体排放是与畜牧业粪便管理相关的主要环境问题。甲烷是粪便露天储存过程中主要的温室气体排放物。本文针对两个养猪场和两个奶牛场的粪便储存罐，从理化特性和微生物学参数两方面对其进行了调查。调查了这些参数在罐间和时空变化上的差异对粪便产甲烷活性的影响。进行了偏最小二乘回归分析，结果表明理化特性和微生物学参数对产甲烷活性有很大影响。在回归分析中包含的 19 个参数中，挥发性脂肪酸（VFAs）的浓度对粪便甲烷排放率的负面影响最大，这是由于它们具有众所周知的抑制作用。在古菌指纹图谱中两个扩增子的相对丰度被发现对产甲烷活性有正向影响，这表明 Methanoculleus spp. 和可能的 Methanosarcina spp. 是储存罐中产甲烷作用的主要贡献者。这项工作深入了解了推动猪粪和奶牛粪储存罐中产甲烷作用的机制。
