Kouritas Vasileios K, Ioannou Maria, Desimonas Nikolaos, Gourgoulianis Konstantinos I, Molyvdas Paschalis A, Hatzoglou Chrisi
Department of Physiology, Medical School, University of Thessaly, Larissa, Greece.
Growth Horm IGF Res. 2013 Feb-Apr;23(1-2):8-12. doi: 10.1016/j.ghir.2012.10.002. Epub 2012 Nov 30.
The effect of IGF-1 in the human pleural permeability and the underlying mechanisms involved were investigated.
Specimens from thoracic surgical patients were mounted in Ussing chambers. Solutions containing IGF-1 (1 nM-100 nM) and IGF-1 Receptor Inhibitor (1 μΜ), amiloride 10 μM (Na(+) channel blocker) and ouabain 1 mM (Na(+)-K(+) pump inhibitor) were used in order to investigate receptor and ion transporter involvement respectively. Trans-mesothelial Resistance (R(TM)) across the pleural membrane was determined as a permeability indicator. Immunohistochemistry for IGF-1 receptors was performed.
IGF-1 increased R(TM) when added on the interstitial surface for all concentrations (p=.008, 1 nM-100 nM) and decreased it on the mesothelial surface for higher concentrations (p=.046, 100 nM). Amiloride and ouabain inhibited this effect. The IGF-1 Receptor Inhibitor also totally inhibited this effect. Immonuhistochemistry demonstrated the presence of IGF-1 receptors in the pleura.
It is concluded that IGF-1 changes the electrophysiology of the human parietal pleura by hindering the normal ion transportation and therefore the pleural fluid recycling process. This event is achieved after IGF-1 interaction with its receptor which is present in the human pleura.
研究胰岛素样生长因子-1（IGF-1）对人胸膜通透性的影响及其潜在机制。
将胸外科手术患者的标本安装在尤斯灌流小室中。分别使用含有IGF-1（1 nM - 100 nM）、IGF-1受体抑制剂（1 μΜ）、10 μM氨氯地平（钠通道阻滞剂）和1 mM哇巴因（钠钾泵抑制剂）的溶液来研究受体和离子转运体的作用。测定跨胸膜的跨间皮电阻（R(TM)）作为通透性指标。进行IGF-1受体的免疫组织化学检测。
所有浓度的IGF-1添加到间质表面时均增加R(TM)（p = 0.008，1 nM - 100 nM），而较高浓度（100 nM）添加到间皮表面时则降低R(TM)（p = 0.046）。氨氯地平和哇巴因抑制了这种作用。IGF-1受体抑制剂也完全抑制了这种作用。免疫组织化学显示胸膜中存在IGF-1受体。
得出结论，IGF-1通过阻碍正常离子转运从而影响胸膜液再循环过程，改变人壁层胸膜的电生理。这一过程是IGF-1与其在人胸膜中存在的受体相互作用后实现的。
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