Kouritas V K, Hatzoglou C, Foroulis C N, Hevas A, Gourgoulianis K I, Molyvdas P A
Dept of Physiology, University of Thessaly Medical School, 22 Papakiriazi St, 412 22, Larissa, Greece.
Eur Respir J. 2007 Aug;30(2):354-7. doi: 10.1183/09031936.00047106.
The aim of the present study was to investigate whether low glucose and pH level, which are usually measured in complicated pleural effusions, alter the electrochemical function of healthy human parietal pleura. Parietal pleural pieces were stripped from 66 patients during thoracic surgery and were mounted in Ussing chambers. Krebs' solutions containing different glucose levels (0, 40 and 100 mg) and balanced at different pH levels (7.4, 7.3 and 7.2) were added to the pleural cavity surface of the pieces. Transmesothelial potential difference was measured at various time-points as an electrophysiological variable and transmesothelial resistance (R(TM)) was calculated using Ohm's law. When normal-glucose Krebs at pH 7.45 was used, R(TM) remained unchanged over time, but when low-glucose Krebs was used, R(TM) decreased. Krebs without glucose caused the greatest decrease in R(TM). Use of low-pH Krebs decreased R(TM). The lower the pH of the Krebs, the faster the decrease in R(TM) and the greater the effect. The decrease in R(TM) was greater with low-pH than with low-glucose Krebs. Low glucose and low pH caused an additive decrease in R(TM). Low glucose concentration and low pH cause alteration of the electrochemical function of human parietal pleura and could act as agents that lead to further exudate progression.
本研究的目的是调查通常在复杂性胸腔积液中测量的低葡萄糖和pH水平是否会改变健康人壁胸膜的电化学功能。在胸外科手术期间从66例患者身上剥离壁胸膜碎片，并将其安装在尤斯灌流小室中。将含有不同葡萄糖水平（0、40和100毫克）并在不同pH水平（7.4、7.3和7.2）下平衡的克雷布斯溶液添加到碎片的胸膜腔表面。在各个时间点测量跨上皮电位差作为电生理变量，并使用欧姆定律计算跨上皮电阻（R(TM)）。当使用pH 7.45的正常葡萄糖克雷布斯溶液时，R(TM)随时间保持不变，但当使用低葡萄糖克雷布斯溶液时，R(TM)降低。不含葡萄糖的克雷布斯溶液导致R(TM)下降幅度最大。使用低pH的克雷布斯溶液会降低R(TM)。克雷布斯溶液的pH越低，R(TM)下降越快，影响越大。低pH克雷布斯溶液导致的R(TM)下降幅度大于低葡萄糖克雷布斯溶液。低葡萄糖和低pH会导致R(TM)的累加性下降。低葡萄糖浓度和低pH会导致人壁胸膜电化学功能的改变，并可能作为导致进一步渗出液进展的因素。
