将蛋白质染成蓝色：β-（1-薁基）-L-丙氨酸作为研究蛋白质-蛋白质相互作用的探针。
Painting proteins blue: β-(1-azulenyl)-L-alanine as a probe for studying protein-protein interactions.
机构信息
Department of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244, USA.
出版信息
Chem Commun (Camb). 2013 Jan 18;49(5):490-2. doi: 10.1039/c2cc37550h.
Abstract
We demonstrated that β-(1-azulenyl)-L-alanine, a fluorescent pseudoisosteric analog of tryptophan, exhibits weak environmental dependence and thus allows for using weak intrinsic quenchers, such as methionines, to monitor protein-protein interactions while not perturbing them.
摘要
我们证明了β-（1-薁基）-L-丙氨酸，色氨酸的荧光假肽类似物，表现出较弱的环境依赖性，因此可以使用较弱的内源性猝灭剂，如蛋氨酸，来监测蛋白质-蛋白质相互作用，而不会干扰它们。
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