Pant Mahendra K, Ahmad Abul H, Naithani Manisha, Pandey Hari S, Pandey Monika, Pant Jayanti
Department of Anatomy, Government Doon Medical College, Dehradun, Uttarakhand 248001, India.
Department of Veterinary Pharmacology & Toxicology, College of Veterinary & Animal Sciences, G.B. Pant University of Agriculture and Technology, Pantnagar, Uttarakhand 263145, India.
Toxics. 2020 May 13;8(2):34. doi: 10.3390/toxics8020034.
Bisphenol A (BPA) is leached out from plastic infant feeding bottles that are filled with warm milk/water due to high temperatures, exposing the infants to BPA. The present study aims to understand the effects of ingestion of BPA leached from plastic infant feeding bottle and delineate the underlying mechanisms in rats. In this study, adult rats of Wistar strain were divided into 3 groups. In the first group, the rats consumed normal food and tap water . In the second group, the rats ingested BPA (20 µg/kg bodyweight/day, orally). In the third group, the rats drank water leached from plastic infant feeding bottles. After 30days, tests involving biochemical parameters, histopathological examination, and oxidative stress enzyme markers were performed, and the levels of BPA in plastic-leached water were estimated by HPLC analysis. There were significant biochemical changes in the form of increased alkaline phosphatase (ALP), creatine kinase-muscle/brain (CK-MB), and lactate dehydrogenase (LDH) levels in both treated groups as compared to control group, accompanied by structural damage to the vital organs, and lipid peroxidation, glutathione reductase, and catalase activity were also high in the treated groups. Further, the BPA concentration in plastic leached water was estimated to be 0.1 ± 0.02 µg/mL.
双酚A（BPA）会从装满温牛奶/水的塑料婴儿奶瓶中因高温而渗出，使婴儿暴露于双酚A中。本研究旨在了解摄入从塑料婴儿奶瓶中渗出的双酚A的影响，并阐明其在大鼠体内的潜在机制。在本研究中，将成年Wistar品系大鼠分为3组。第一组大鼠食用正常食物并饮用自来水。第二组大鼠摄入双酚A（20微克/千克体重/天，经口）。第三组大鼠饮用从塑料婴儿奶瓶中渗出的水。30天后，进行了涉及生化参数、组织病理学检查和氧化应激酶标志物的测试，并通过高效液相色谱分析估计了塑料渗出水中双酚A的含量。与对照组相比，两个处理组均出现了显著的生化变化，表现为碱性磷酸酶（ALP）、肌酸激酶-肌肉/脑型（CK-MB）和乳酸脱氢酶（LDH）水平升高，同时伴有重要器官的结构损伤，并且处理组中的脂质过氧化、谷胱甘肽还原酶和过氧化氢酶活性也较高。此外，估计塑料渗出水中双酚A的浓度为0.1±0.02微克/毫升。
