完整的嵌合体基因组序列是通过将集胞藻 PCC6803 全基因组克隆到枯草芽孢杆菌 168 基因组中构建的。
Complete sequence of the first chimera genome constructed by cloning the whole genome of Synechocystis strain PCC6803 into the Bacillus subtilis 168 genome.
机构信息
Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan.
出版信息
J Bacteriol. 2012 Dec;194(24):7007. doi: 10.1128/JB.01798-12.
Abstract
Genome synthesis of existing or designed genomes is made feasible by the first successful cloning of a cyanobacterium, Synechocystis PCC6803, in Gram-positive, endospore-forming Bacillus subtilis. Whole-genome sequence analysis of the isolate and parental B. subtilis strains provides clues for identifying single nucleotide polymorphisms (SNPs) in the 2 complete bacterial genomes in one cell.
摘要
通过在革兰氏阳性、产芽孢的枯草芽孢杆菌中首次成功克隆蓝藻集胞藻 PCC6803，使得对现有或设计的基因组进行基因组合成成为可能。对分离株和亲本枯草芽孢杆菌菌株的全基因组序列分析为在一个细胞中鉴定 2 个完整细菌基因组中的单核苷酸多态性（SNP）提供了线索。
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