Faculty of Biology, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany.
DNA Res. 2012 Dec;19(6):435-48. doi: 10.1093/dnares/dss024. Epub 2012 Oct 15.
Synechocystis sp. PCC 6803 is a widely used model cyanobacterium for studying photosynthesis, phototaxis, the production of biofuels and many other aspects. Here we present a re-sequencing study of the genome and seven plasmids of one of the most widely used Synechocystis sp. PCC 6803 substrains, the glucose tolerant and motile Moscow or 'PCC-M' strain, revealing considerable evidence for recent microevolution. Seven single nucleotide polymorphisms (SNPs) specifically shared between 'PCC-M' and the 'PCC-N and PCC-P' substrains indicate that 'PCC-M' belongs to the 'PCC' group of motile strains. The identified indels and SNPs in 'PCC-M' are likely to affect glucose tolerance, motility, phage resistance, certain stress responses as well as functions in the primary metabolism, potentially relevant for the synthesis of alkanes. Three SNPs in intergenic regions could affect the promoter activities of two protein-coding genes and one cis-antisense RNA. Two deletions in 'PCC-M' affect parts of clustered regularly interspaced short palindrome repeats-associated spacer-repeat regions on plasmid pSYSA, in one case by an unusual recombination between spacer sequences.
集胞藻 PCC 6803 是一种广泛用于研究光合作用、趋光性、生物燃料生产和许多其他方面的模式蓝藻。在这里，我们对最广泛使用的集胞藻 PCC 6803 亚株之一，即耐葡萄糖和运动性的莫斯科或“PCC-M”株的基因组和七个质粒进行了重新测序研究，揭示了最近微进化的大量证据。七个单核苷酸多态性（SNP）仅在“PCC-M”和“PCC-N 和 PCC-P”亚株之间共享，表明“PCC-M”属于运动性菌株的“PCC”组。在“PCC-M”中鉴定出的插入缺失和 SNP 可能影响葡萄糖耐受性、运动性、噬菌体抗性、某些应激反应以及初级代谢中的功能，这些功能可能与烷烃的合成有关。两个位于基因间区的 SNP 可能影响两个蛋白编码基因和一个顺式反义 RNA 的启动子活性。“PCC-M”中的两个缺失影响了质粒 pSYSA 上簇状规则间隔短回文重复相关间隔重复区的部分区域，在一种情况下，通过间隔序列之间的异常重组。
