Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University, 1, 5-ga, Anam-dong, Sungbuk-ku, Seoul 136-701, South Korea.
Food Chem Toxicol. 2013 Mar;53:214-20. doi: 10.1016/j.fct.2012.11.032. Epub 2012 Dec 2.
Lindera obtusiloba Blume, a native plant of East Asia, has traditionally been used as a folk medicine for liver disease. We studied the in vitro antioxidant and in vivo hepatoprotective activities of a 70% ethanolic extract of L. obtusiloba (LOE) containing 62.9% quercitrin and 22.0% afzelin. LOE prevented tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in HepG2 cells. Along with its high antioxidant potency in vitro, our animal study confirmed that pretreatment with LOE (500 or 2000 mg/kg) for 7 days prior to a single dose of t-BHP (i.p.: 0.5 mmol/kg) significantly lowered the serum levels of alanine and aspartate aminotransferases. In addition, glutathione levels were increased in the liver, and lipid peroxidation levels were decreased in a dose-dependent manner. The histopathological examinations of rat livers showed that LOE significantly reduced the incidence of liver lesions induced by t-BHP. Therefore, we concluded that LOE has merit as a potent candidate to protect the liver against oxidative damage.
钝叶桂，一种原产于东亚的植物，传统上被用作治疗肝脏疾病的民间药物。我们研究了含有 62.9%槲皮素和 22.0%山柰酚的 70%乙醇提取物（LOE）的体外抗氧化和体内保肝活性。LOE 可预防 HepG2 细胞中二丁基过氧化物（t-BHP）诱导的氧化损伤。我们的动物研究证实，LOE（500 或 2000 mg/kg）预处理 7 天，然后单次腹腔注射 t-BHP（0.5 mmol/kg），可显著降低血清丙氨酸和天冬氨酸转氨酶水平。此外，LOE 还可剂量依赖性地增加肝脏中的谷胱甘肽水平，并降低脂质过氧化水平。大鼠肝脏的组织病理学检查表明，LOE 可显著降低 t-BHP 诱导的肝损伤发生率。因此，我们得出结论，LOE 作为一种有效的候选药物，具有保护肝脏免受氧化损伤的作用。
