Kang Sini, Johnston Tony V, Ku Seockmo, Ji Geun Eog
Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University, Seoul 08826, Korea.
Fermentation Science Program, School of Agriculture, College of Basic and Applied Sciences, Middle Tennessee State University, Murfreesboro, TN 37132, USA.
Toxicol Res (Camb). 2020 Jul 27;9(4):484-492. doi: 10.1093/toxres/tfaa055. eCollection 2020 Jul.
B-FOS (butyl-fructooligosaccharide) is a newly synthesized prebiotic molecule, formed by the combination of FOS and butyrate by ester bonds. B-FOS has been reported to have the potential prebiotic effect of promoting intestinal flora diversity and enhancing butyrate production. The aim of this study was to investigate the potential acute and sub-chronic toxicity of B-FOS in Institute of Cancer Research (ICR) mice and Wistar rats to verify its biosafety. ICR mice were administered a single oral gavage of B-FOS at doses of 0, 500, 1000, and 2000 mg/kg body weight and observed for signs of acute toxicity for 14 days. Sub-chronic toxicity was evaluated by repeated oral administration of B-FOS at 2000 mg/kg for 28 days, in accordance with Organization for Economic Co-operation and Development (OECD) protocol test numbers 420 and 407. No mortality or abnormal clinical signs were observed during the experimental periods after B-FOS administration. Furthermore, no significant changes in body weight, organ weight, serum biochemical parameters, or tissue histology were observed after animal sacrifice. These results indicate that B-FOS does not exert any acute or sub-chronic toxicity at a dose of 2000 mg/kg, and this novel molecule can be regarded as a safe prebiotic substance for use in the food and nutraceutical industries.
丁酰基低聚果糖（B-FOS）是一种新合成的益生元分子，由低聚果糖（FOS）和丁酸盐通过酯键结合而成。据报道，B-FOS具有促进肠道菌群多样性和提高丁酸盐产量的潜在益生元效应。本研究的目的是调查B-FOS对癌症研究所（ICR）小鼠和Wistar大鼠的潜在急性和亚慢性毒性，以验证其生物安全性。给ICR小鼠单次经口灌胃给予剂量为0、500、1000和2000毫克/千克体重的B-FOS，并观察14天的急性毒性迹象。根据经济合作与发展组织（OECD）试验编号420和407的方案，通过以2000毫克/千克的剂量重复口服B-FOS 28天来评估亚慢性毒性。在给予B-FOS后的实验期间，未观察到死亡或异常临床体征。此外，在动物处死后，未观察到体重、器官重量、血清生化参数或组织组织学有显著变化。这些结果表明，B-FOS在2000毫克/千克的剂量下不会产生任何急性或亚慢性毒性，这种新型分子可被视为用于食品和营养保健品行业的安全益生元物质。
