Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan 610041, PR China.
Bioresour Technol. 2013 Jan;128:560-4. doi: 10.1016/j.biortech.2012.09.134. Epub 2012 Oct 16.
Viscosity reduction and the effect of cell-wall degrading enzymes (CWDEs) were investigated using Canna edulis Ker. for bioethanol fermentation. The fermentation mash treated with CWDEs was much thinner (2.12 Pas) than the control mash (8.42 Pas), the fermentation efficiency was increased from 90.46% to 96.11%. HPLC analysis revealed that after treated with CWDEs, glucose and total sugar were increased by 28.07% and 7.60%, respectively. Changes in the starch granules were investigated by scanning electron microscopy (SEM), atomic force microscopy (AFM), and confocal laser scanning microscopy (CLSM). The results suggested that the reduction in viscosity was caused by changes in saccharide composition and physical changes of the starch granules. This present study is of significance that non-starch polysaccharides and starch can be simultaneously utilized for bioethanol production using roots and tubers as feedstock.
采用美人蕉（Canna edulis Ker.）进行生物乙醇发酵，研究了黏度降低和细胞壁降解酶（CWDEs）的作用。经 CWDE 处理的发酵醪比对照醪（8.42Pas）更稀（2.12Pas），发酵效率从 90.46%提高到 96.11%。HPLC 分析表明，经 CWDE 处理后，葡萄糖和总糖分别增加了 28.07%和 7.60%。通过扫描电子显微镜（SEM）、原子力显微镜（AFM）和共聚焦激光扫描显微镜（CLSM）研究了淀粉颗粒的变化。结果表明，黏度降低是由于糖的组成变化和淀粉颗粒的物理变化引起的。本研究的意义在于，可以利用根茎类作物作为原料，同时利用非淀粉多糖和淀粉进行生物乙醇生产。
