Huang Yuhong, Willats William G, Lange Lene, Jin Yanling, Fang Yang, Salmeán Armando A, Pedersen Henriette L, Busk Peter Kamp, Zhao Hai
Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, People's Republic of China.
University of the Chinese Academy of Sciences, Beijing, People's Republic of China.
Biotechnol Appl Biochem. 2016 Mar-Apr;63(2):178-89. doi: 10.1002/bab.1367.
Viscosity reduction has a great impact on the efficiency of ethanol production when using roots and tubers as feedstock. Plant cell wall-degrading enzymes have been successfully applied to overcome the challenges posed by high viscosity. However, the changes in cell wall polymers during the viscosity-reducing process are poorly characterized. Comprehensive microarray polymer profiling, which is a high-throughput microarray, was used for the first time to map changes in the cell wall polymers of sweet potato (Ipomoea batatas), cassava (Manihot esculenta), and Canna edulis Ker. over the entire viscosity-reducing process. The results indicated that the composition of cell wall polymers among these three roots and tubers was markedly different. The gel-like matrix and glycoprotein network in the C. edulis Ker. cell wall caused difficulty in viscosity reduction. The obvious viscosity reduction of the sweet potato and the cassava was attributed to the degradation of homogalacturonan and the released 1,4-β-d-galactan and 1,5-α-l-arabinan.
以块根和块茎为原料生产乙醇时，降低粘度对生产效率有很大影响。植物细胞壁降解酶已成功应用于克服高粘度带来的挑战。然而，在粘度降低过程中细胞壁聚合物的变化却鲜有报道。首次使用综合微阵列聚合物分析（一种高通量微阵列）来描绘甘薯（Ipomoea batatas）、木薯（Manihot esculenta）和食用美人蕉（Canna edulis Ker.）在整个粘度降低过程中细胞壁聚合物的变化。结果表明，这三种块根和块茎的细胞壁聚合物组成明显不同。食用美人蕉细胞壁中的凝胶状基质和糖蛋白网络导致粘度降低困难。甘薯和木薯的明显粘度降低归因于同型半乳糖醛酸的降解以及释放的1,4-β-D-半乳聚糖和1,5-α-L-阿拉伯聚糖。
