Huang Yuhong, Jin Yanling, Shen Weiliang, Fang Yang, Zhang Guohua, Zhao Hai
Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, People's Republic of China.
Graduate University of the Chinese Academy of Sciences, Beijing, People's Republic of China.
Biotechnol Appl Biochem. 2014 Jul-Aug;61(4):480-91. doi: 10.1002/bab.1190. Epub 2014 Jun 9.
Penicillium ochrochloron Biourge, which was isolated from rotten sweet potato, can produce plant cell wall-degrading enzymes (PCWDEs) with high viscosity reducing capability for ethanol production using fresh sweet potato tubers as feedstock. The enzyme preparation was characterized by a broad enzyme spectrum including 13 kinds of enzymes with the activity to hydrolyze cellulose, hemicellulose, pectin, starch, and protein. The maximum viscosity-reducing capability was observed when the enzyme preparation was obtained after 5 days of fermentation using 20 g/L corncob as a sole carbon source, 4.5 g/L NH4 NO3 as a sole nitrogen source, and an initial medium pH of 6.5. The sweet potato mash treated with the enzyme preparation exhibited much higher fermentation efficiency (92.58%) compared with commercial cellulase (88.06%) and control (83.5%). The enzyme production was then scaled up to 0.5, 5, and 100 L, and the viscosity-reducing rates were found to be 85%, 90%, and 91%, respectively. Thus, P. ochrochloron Biourge displays potential viscosity-reducing capability for ethanol production.
从腐烂红薯中分离得到的黄绿青霉（Penicillium ochrochloron Biourge），能够产生植物细胞壁降解酶（PCWDEs），该酶对以新鲜红薯块茎为原料生产乙醇具有很高的降黏能力。该酶制剂具有广泛的酶谱，包括13种具有水解纤维素、半纤维素、果胶、淀粉和蛋白质活性的酶。当以20 g/L玉米芯作为唯一碳源、4.5 g/L硝酸铵作为唯一氮源、初始培养基pH值为6.5发酵5天后获得酶制剂时，观察到最大降黏能力。用该酶制剂处理的红薯醪液与商业纤维素酶（88.06%）和对照（83.5%）相比，发酵效率更高（92.58%）。然后将酶产量扩大到0.5、5和100 L，发现降黏率分别为85%、90%和91%。因此，黄绿青霉在乙醇生产中显示出潜在的降黏能力。
