Fujinaga M, Baden J M, Shepard T H, Mazze R I
Department of Anesthesia, Stanford University School of Medicine, California 94305.
Teratology. 1990 Feb;41(2):131-5. doi: 10.1002/tera.1420410202.
Seventy timed-pregnant Sprague-Dawley rats were exposed to either air (control) or 75% nitrous oxide (N2O) for 24 hours on day 8 of gestation. Four rats from each group were killed on days 11-16, 18, and 20, and laparotomy was performed. The viability of the embryos/fetuses was determined, as was the side of tail flexion on days 11 and 12, the direction from which the umbilical artery emerged from the body on days 13 and 14, the side of the body facing the placenta on days 15 and 16, and the side to which the aortic arch curved on days 18 and 20. Mean mortality rate in the control group was 8.9 +/- 6.1% (+/- S.D.), and there were no control embryos/fetuses with altered laterality except the 9% that faced left on day 16. In contrast, N2O treatment on day 8 of gestation resulted in significantly increased mortality (40.8 +/- 3.3%) beginning on day 14 of gestation and increased incidence of altered laterality overall (31.3%) and at all stages of development. The mechanisms underlying these events remain to be defined, as do the implications of our findings for pregnant surgical patients and occupationally exposed workers.
70只处于孕期特定阶段的斯普拉格-道利大鼠在妊娠第8天被暴露于空气（对照组）或75%氧化亚氮（N2O）环境中24小时。每组各4只大鼠分别在妊娠第11 - 16天、第18天和第20天被处死，并进行剖腹手术。确定胚胎/胎儿的存活率，以及在第11天和第12天尾屈的方向、在第13天和第14天脐动脉从身体发出的方向、在第15天和第16天身体朝向胎盘的一侧，以及在第18天和第20天主动脉弓弯曲的一侧。对照组的平均死亡率为8.9 +/- 6.1%（+/-标准差），除了第16天9%的胚胎/胎儿面朝左侧外，没有对照组胚胎/胎儿出现侧性改变。相比之下，妊娠第8天进行氧化亚氮处理导致从妊娠第14天开始死亡率显著增加（40.8 +/- 3.3%），并且总体侧性改变的发生率增加（31.3%），在所有发育阶段均如此。这些事件背后的机制以及我们的发现对妊娠手术患者和职业暴露工人的影响仍有待确定。
