Department of Evolutionary Immunology, Jagiellonian University, Gronostajowa 9, PL30-387, Krakow, Poland.
Gen Comp Endocrinol. 2013 Jul 1;188:102-9. doi: 10.1016/j.ygcen.2012.11.014. Epub 2012 Dec 2.
Inflammatory responses have to be carefully controlled, as high concentrations and/or prolonged action of inflammation-related molecules (e.g. reactive oxygen species, nitric oxide and pro-inflammatory cytokines) can be detrimental to host tissue and organs. One of the potential regulators of the inflammatory process are stress mediators including adrenaline. In vivo effects of adrenaline were studied during zymosan-induced (Z) peritoneal inflammation in the common carp Cyprinus carpio L. Adrenaline injected together with zymosan (ZA) did not change the number of inflammatory leukocytes in the peritoneal cavity, however at 24h post-injection it significantly reduced the percentage of monocytes/macrophages. Moreover, compared to cells retrieved from fish treated with PBS or zymosan only, adrenaline increased the percentage of apoptotic leukocytes in the focus of inflammation. Furthermore, adrenaline significantly reduced the expression of chemokine CXCL8_L1 (a functional homolog of mammalian IL-8) and its receptors (CXCR1 and CXCR2), indicating changes in leukocyte recruitment after stress. We conclude that adrenaline may contribute to a coordinated reaction by influencing the inflammatory response via direct regulation of leukocyte migration and/or apoptosis.
炎症反应必须得到仔细控制，因为高浓度和/或长时间的炎症相关分子（如活性氧、一氧化氮和促炎细胞因子）作用可能对宿主组织和器官有害。应激介质包括肾上腺素，是炎症过程的潜在调节剂之一。在鲤鱼（Cyprinus carpio L.）的酵母聚糖（Z）诱导的腹膜炎症中研究了肾上腺素的体内作用。与酵母聚糖一起注射的肾上腺素（ZA）并没有改变腹腔中的炎症白细胞数量，但在注射后 24 小时，它显著降低了单核细胞/巨噬细胞的百分比。此外，与仅用 PBS 或酵母聚糖处理的鱼类中回收的细胞相比，肾上腺素增加了炎症焦点中凋亡白细胞的百分比。此外，肾上腺素显著降低了趋化因子 CXCL8_L1（哺乳动物 IL-8 的功能同源物）及其受体（CXCR1 和 CXCR2）的表达，表明应激后白细胞募集的变化。我们得出结论，肾上腺素可能通过直接调节白细胞迁移和/或凋亡来影响炎症反应，从而有助于协调反应。
